Magnetic nanoparticle based purification and enzyme-linked immunosorbent assay using monoclonal antibody against enrofloxacin

Monoclonal anti-enrofloxacin antibody was prepared for a direct competitive enzyme-linked immunosorbent assay (ELISA) and purification system using monoclonal antibody (mAb) coupled magnetic nanoparticles (MNPs). The IC50 values of the developed mAb for enrofloxacin (ENR), ciprofloxacin, difloxacin, sarafloxacin, pefloxacin, and norfloxacin were 5.0, 8.3, 9.7, 21.7, 36.0, and 63.7 ng/mL, respectively. The lowest detectable level of ENR was 0.7 ng/mL in the prepared ELISA system. To validate the developed ELISA in the food matrix, known amounts of ENR were spiked in meat and egg samples at 10, 20 and 30 ng/mL. Recoveries for ENR ranged from 72.9 to 113.16% with a coefficient of variation (CV) of 2.42 to 10.11%. The applicability of the mAb-MNP system was verified by testing the recoveries for ENR residue in three different matrices. Recoveries for ENR ranged from 75.16 to 86.36%, while the CV ranged from 5.08 to 11.53%. Overall, ENR-specific monoclonal antibody was prepared and developed for use in competitive to ELISAs for the detection of ENR in animal meat samples. Furthermore, we suggest that a purification system for ENR using mAb-coupled MNPs could be useful for determination of ENR residue in food.

Molecular transport mechanism of pefloxacin mesylate binding with transferrin / 药学学报

The binding mechanism between pefloxacin mesylate (PM) and transferrin (Tf) was explored using spectral experiment combined with molecular modeling techniques. The binding parameters and thermodynamic functions of PM-Tf solution system were measured at different temperatures. The effect of PM on molecular conformation of Tf was investigated and the interaction mechanism was also discussed. The results showed that dynamic quenching mechanism occurs with PM binding to Tf. The value of binding distances (r) is low, which indicates the occurrence of energy transfer. The drug had conformational effect on Tf, which resulted in changes of hydrophobic environment of the binding domain in Tf. According to the obtained thermodynamic parameters, the main interaction force between PM and Tf is attributed to hydrophobic bonding. The results of molecular modeling revealed that hydrophobic and hydrogen bonds are main binding forces in the PM-Tf system. These results were in accordance with spectral experiments. The research results have given a better theoretical reference for the study of pharmacological mechanism between protein and quinolone.

[ harmful effect of quinolone [pefloxacine] on immature cartilage in juvenile rat]

The aim of our study was definition of the exact mechanism by which quinolone harm the immature cartilage causing arthropathy, and upon these results we can determine the suitable treatment for this damage. This effect was studied in four groups of juvenile wistar rats as following:1- normal group.2- the group of pefloxacine with a dose 15 mg/kg per day for one month. 3- the group of pefloxacine 15mg/kg per day for one month with addition of the magnesium ions with compensate amount 84mg/kg per art. 4- the group of pefloxacine 15mg/kg per day for one month with addition of vitamin E with compensate amount75mg/kg per rat. The drugs were administered by incubation tube for one months, and at the end of the experiment blood samples were took from animals hearts preparing for measuring malondialdehide, then animals were scarified and the maximum extension angle of the knee joints was measured, then we put the joints in the formol for the histological study. Pefloxacine increased the values of maximum extension angle significantly in compare to the normal group and the control group, with significant improvement in the group of magnesium ions and the group of vitamin E but non of each group could return the cartilage to the normal condition. The results of malondiadehyde assay inssure the effect of free radicals in this arthropathy. Histological study: pefloxacine damage significantly the cartilage and the damage was decreased in the addition of magnesium or vitamin E. There is a lot of factors participate in causing the arthropathy which induced by taking quinolone and this pathology don't confine on the participate of magnesium ions or oxidative stress.

Validation of UV Spectrophotometric Method of Analysis of some Marketed Perfloxacin in Nigeria

Background: Acquiring sophisticated LC instruments by most third world laboratories is capital intensive.Literatures on simple spectrophotometric analytical methods for pefloxacin are scarce. Objectives: The present study was undertaken to develop and validate a simple and economic UV spectrophotometric method for estimating pefloxacin mesylate (PFM) in dosage preparations.Methods: Using a JENWAY spectrophotometer at predetermined emax of 277nm with 1 v/v aqueous glacialacetic acid as blank; the method was validated for linearity; accuracy; precision; reproducibility; and specificity asper International Conference on Harmonization (ICH) guidelines and used to determine the content of pefloxacinin seven marketed brands in Nigeria. Results: The method exhibited good linearity over a range of 0.40-12.0 ?g/ml (regression equation: y = 0.0859x+0.0211 ; r=0.999). Mean recovery accuracy (99.183 ) and assay result in the range of 100.5- 110.17 for these lected brands were not significantly different at p=0.05. The coefficient of variation (CV) for both intra andinter-day were below 7 . The method was specific for pefloxacin in the presence of common excipients Conclusion: The method gave good validation results and could be employed for routine analysis of PFM incommercial formulations

Teratogenic effects of ciprofloxacin and pefloxacin in pregnant rats

The adverse effects of ciprofloxacin and pefloxacin on rat fetuses following oral administration to the pregnant rats were studied. Ciprofloxacin and pefloxacin were given daily to pregnant rats at two [therapeutic and double therapeutic] dosage levels during the period of organogenesis [from the 6[th] to the 15[th] of gestation]. Oral administration of ciprofloxacin at 4.5 and 9 mg/100 g.b.wt. to pregnant rats induced fetal resorption, death and growth retardation. Visceral examination of live fetuses showed microcephaly, hypoplasia of the heart and lungs and dilatation of renal pelvis. Skeletal examination reveated incomplete ossification of skull bones and absence of some coccygeal vertebrae. Pefloxacin at 7.2 6 mg/100 g. b. wt. decrease the number of live fetuses and caused microcephaly, hypoplasia of the heart and lungs, dilatation of the renal pelvis. It also induced incomplete ossification of skull bones and absence of sternbrae and phalanges of forelimbs. A great attention should be considered during the clinical use of ciprofloxacin and pefloxacin in pregnant women and animals to avoid their teratogenic effects

Ùlcera crônica de perna de pacientes em tratamentoambulatorial: análise microbiológica e desuscetibilidade antimicrobiana / Chronic leg ulcer patients in outpatient treatment: analysis ofmicrobiological and antimicrobial susceptibility / Ùlcera crónica de pierna de pacientes en tratamiento ambulatorio: análisis microbiológico y de susceptibilidad antimicrobiana

A ocorrência de microrganismos em feridas crônicas varia geograficamente e o perfil de suscetibilidade antimicrobiana requer monitoramento para fornecer informações que possam subsidiar opções terapêuticas econdutas no atendimento. O objetivo deste estudo foi isolar e identificar microrganismos de úlceras crônicas deperna em pacientes ambulatoriais e verificar a suscetibilidade antimicrobiana dos agentes etiológicos. O presenteestudo é de caráter transversal e foi realizado em unidades básicas de saúde de Goiânia, com atendimento de24 horas. Os dados foram obtidos por meio de entrevista estruturada e de um check-list contendo sinais e sintomas clínicos indicativos de infecção e amostra por meio de swab e técnica de Levine. A coleta de dados foirealizada no período de junho a julho de 2007. Participaram 46 pacientes que apresentavam 60 feridas. Entre asbactérias gram-positivas predominou, em 65 por cento dos casos, Staphylococcus aureus sensível à maioria dos antibióticos testados. Entre as gram-negativas, as mais frequentes foram: Pseudomonas aeruginosa (23,3 por cento), resistente a amoxicilina+ácido clavulânico, cefalexina e cefotaxima; Proteus mirabilis (16,6 por cento) e Proteus vulgaris(15,0 por cento) foram sensíveis à gentamicina, ao aztreonam, ao ciprofloxacino e à amicacina. A caracterização dos isolados evidencia a necessidade de monitoramento dos agentes, em vista da possibilidade de disseminação e ocorrência de surtos e infecções por estas bactérias.

The occurrence of microorganisms in chronic wounds vary geographically and antimicrobial susceptibility requires close monitoring to provide information that may support and offer therapeutic options to assist individuals with chronic injuries. The objective of this study was to isolate and to identify microorganisms from chronic leg ulcers inoutpatients and to verify the antimicrobial susceptibility of etiological agents. This is a cross-sectional studycarried out in Basic Health Units of Goiania, with 24 hours attendance. Data were collected through structured interview and a check-list containing clinical signs and symptoms indicative of infection and sample collected by swab and Levine technique. Data collection was performed from June to July 2007. Took part on the study 46patients totaling 60 wounds. Among the Gram-positive bacteria Staphylococcus aureus were predominant, present in 65 percent of cases and were sensitive to most antibiotics tested. Among the Gram-negative bacteria, themost frequent were: Pseudomonas aeruginosa (23.35), resistant to amoxicillin + clavulanic acid, cephalexin and cefotaxime; Proteus mirabilis (16.6 percent) and Proteus vulgaris (15.0 percent) all sensitive to gentamicin, aztreonam,ciprofloxacin and amikacin. The characterization of the isolates indicate the need for monitoring of the agents because the possibility of spreading, outbreaks and infections by these bacteria.

La ocurrencia de microorganismos en heridas crónicas varía geográficamente y el perfil de susceptibilidad antimicrobiana requiere una estrecha supervisión para ofrecer informaciones que puedan subvencionar opciones terapéuticas y conductas en la atención. El objetivo de este estudio fue aislar e identificar microorganismos de úlceras crónicas de pierna en pacientes ambulatoriales y verificar la susceptibilidad antimicrobiana de losagentes etiológicos. El presente estudio es de carácter transversal y fue realizado en Unidades Básicas de Saludde Goiania, con atención 24 horas. Los datos fueron recolectados a través de entrevista estructurada y de uncheck-list conteniendo señales y síntomas clínicos indicativos de infección y muestra por medio de swab ytécnica de Levine. La recogida de datos fue realizada de junio a julio de 2007. Participaron 46 pacientes que presentaban 60 heridas. Entre las bacterias gram-positivas predominó, en 65 por ciento de los casos, Staphylococcusaureus sensible a la mayoría de los antibióticos testados. Entre las bacterias gram-negativas, las más frecuentesfueron: Pseudomonas aeruginosa (23,3 por ciento), resistentes a amoxicilina + ácido clavulánico, cefalexina y cefotaxima,Proteus mirabilis (16,6 por ciento) y Proteus vulgaris (15,0 por ciento) sensibles a la gentamicina, aztreonam, ciprofloxacina yamikacina. La caracterización de los aislados indica la necesidad de un control de los agentes, en vista de laposibilidad de diseminación y ocurrencia de surtos e infecciones por estas bacterias.

Development and validation of a HPLC method for determination of pefloxacin in tablet and human plasma

Developing and validating a simple, efficient, reproducible and economic reversed phase high performance liquid chromatographic [RP-HPLC] method for the quantitative determination of pefloxacin in bulk material, tablets and in human plasma. A shim-pack CLC-ODS column and a mobile phase constituting acetonitrile: 0.025 M phosphoric acid solution [13:87 v/v, pH 2.9 adjusted with KOH] was used. The flow rate was 1 ml/min and the analyses performed using ultraviolet [UV] detector at a wavelength of 275 nm using acetaminophen as an internal standard. The developed method showed good resolution between pefloxacin and acetaminophen. It was selective to pefloxacin and able to resolve the drug peak from internal standard and from formulation excipients. The percentage of coefficient variation [CV] of the retention times and peak areas of pefloxacin from the six consecutive injections were 0.566% and 0.989%, respectively. The results showed that the peak area responses are linear within the concentration range of 0.125 mg/ml-12 mg/ml [R2=0.9987]. The limits of detection [LOD] and limits of quantitation [LOQ] for pefloxacin were 0.03125 mg/ml and 0.125 mg/ml. The intra-day and inter-day variation, RSD were 0.376-0.9056 and 0.739-0.853 respectively; also, inter-day variation with relative standard deviation [RSD] were 0.1465-0.821 in plasma. The accuracy results of 70%, 100%, and 130% drugs were 100.72%, 100.34%, and 100.09%, respectively. The method is linear, quantitative, reproducible and could be used as a more convenient, efficient and economical method for the trace analysis of drug in biological fluids, in raw material and tablets

Effect of trikatu pretreatment on the pharmacokinetics of pefloxacin administered orally in mountain Gaddi goats

The pharmacokinetics of orally administered pefloxacin were studied to evaluate the bio-enhancing effect of the herbal bio-enhancer, trikatu, in mountain Gaddi goats (n = 6). The findings of the study revealed a decreased plasma concentration (p > 0.05) of pefloxacin following trikatu administration during the absorption phase (10, 15, 20 min post pefloxacin administration). In contrast, the plasma concentrations of pefloxacin were significantly higher at 4, 6, 8 and 12 h (during the elimination phase) of the pefloxacin administration. The findings of the investigation revealed higher values for the area under the curve, the area under the first moment of the plasma drug concentration time curve, the mean residential time, the total duration of pharmacological action and bioavailability. Trikatu treatment, however, significantly reduced the elimination half life (t(1/2beta)) and zero time intercept of the elimination phase. The apparent volume of distribution based on the total area under the plasma drug concentration curve [(Vd((area))] and the apparent volume of distribution based on the zero time plasma concentration intercept of the elimination phase [Vd((B))] were significantly higher in trikatu treated animals indicating a better penetration of the drug. Based on the MIC of 0.8 microgram/ml of pefloxacin, a priming dose of 6.0 mg/kg and a maintenance dose of 2.21 mg/kg is required to be administered at 8 h intervals. For practical purposes in goats this would mean a priming dose of 6 mg/kg and a maintenance dose of 2 mg/kg given by the oral route, to be repeated at 8 h intervals.

Binding interactions of pefloxacin mesylate with bovine lactoferrin and human serum albumin / 浙江大学学报（英文版）（B辑：生物医学和生物技术）

The binding of pefloxacin mesylate (PFLX) to bovine lactoferrin (BLf) and human serum albumin (HSA) in dilute aqueous solution was studied using fluorescence spectra and absorbance spectra. The binding constant K and the binding sites n were obtained by fluorescence quenching method. The binding distance r and energy-transfer efficiency E between pefloxacin mesylate and bovine lactoferrin as well as human serum albumin were also obtained according to the mechanism of Förster-type dipole-dipole nonradiative energy-transfer. The effects of pefloxacin mesylate on the conformations of bovine lactoferrin and human serum albumin were also analyzed using synchronous fluorescence spectroscopy.

The Effect of Pefloxacin on Rocuronium-Induced Neuromuscular Blockade in Rat Phrenic Nerve-Hemidiaphragm Preparations / 대한마취과학회지

BACKGROUND: At the neuromuscular junction, pefloxacin (P) may exacerbate myasthenia gravis and reduce the tau of MEPC. So here we investigated the effect of P on the neuromuscular blocking action of rocuronium (R). METHODS: Hemidiaphragm-phrenic nerve preparations were obtained from male Sprague-Dawley rats (150-250 g). Preparations were bathed in Kreb's solution (in (mM): NaCl 118, KCl 5, CaCl2 2.5, NaHCO3 30, KH2PO4 1, MgCl2 1 and glucose 11), maintained at 32oC and then aerated with a mixture of 95% O2 and 5% CO2. Isometric forces generated in response to 0.1 Hz, and, 50 Hz for 19 seconds with supramaximal electrical stimulation(0.2 msec, rectangular) to the phrenic nerve, were measured with a force transducer. Single twitch tension (ST) and peak tetanic tension (PTT) were calculated as % reduction versus the control, and tetanic fade (TF), as a % increase. Each preparation was exposed to one of 4 P concentrations of Krebs' solution (0, 0.25, 0.5, 1.0 mM), and enough R solution was added to the tissue bath to achieve the desired R concentration. The effects of P and R were allowed to stabilize before measuring tension parameters. EC5, EC25, EC50, EC75, and EC95 of R for ST, PTT and TF were calculated using a probit model. The interactions between the two drugs were drawn with Berenbaum's additive isobole at 25% isobole, 50% isobole, and 75% isobole. Differences between EC50's of R according to P concentrations were tested by one way ANOVA with Tamhane for post hoc; P <0.05 was regarded as significant. RESULTS: The cumulative concentration-effect curves shifted to the right in ST, and to the left in TF as the concentration of P was increased. The interactions between these two drugs varied from additive to antagonistic according to the magnitude of relaxation effect, drug concentration, and the frequency of stimulation. CONCLUSIONS: P augmented the TF of R. Our results suggest that simultaneous 0.1 Hz and 50 Hz stimulations allow the neuromuscular blocking action of a drug to be correctly evaluated.

The effect of nitric oxide combined with fluoroquinolones against Salmonella enterica serovar Typhimurium in vitro

Two regulons, soxRS and marRAB, are associated with resistance to quinolones or multiple antibiotic in Salmonella enterica serovar Typhimurium. These regulons are activated by nitric oxide and redox-cycling drugs, such as paraquat and cause on activation of the acrAB-encoded efflux pump. In this study, we investigated the effect of nitric oxide (NO) alone and in combination with ofloxacin, ciprofloxacin, and pefloxacin against S. typhimurium clinical isolates and mutant strains in vitro. We did not observe synergistic effect against clinical isolates and SH5014 (parent strain of acr mutant), while we found synergistic effect against PP120 (soxRS mutant) and SH7616 (an acr mutant) S. typhimurium for all quinolones. Our results suggest that the efficiencies of some antibiotics, including ofloxacin, ciprofloxacin, and pefloxacin are decreased via activation of soxRS and marRAB regulons by NO in S. enterica serovar Typhimurium. Further studies are warranted to establish the interaction of NO with the genes of Salmonella and, with multiple antibiotic resistance

Neuromuscular Blocking Actions and Mechanisms of Kanamycin, Pefloxacin and Rocuronium in the Rat Phrenic Nerve-Hemidiaphragm Preparations / 대한마취과학회지

BACKGORUND: Kanamycin (K) has been shown to block neuromuscular transmission by reducing acetylcholine release or postsynaptic action. Pefloxacin (P) may exacerbate myasthenia gravis and reduce the tau of MEPP. Rocuronium (R) is still the subject of dispute as to whether it has a selective presynaptic effect. Therefore, we undertook to compare the muscle relaxation actions and reversibilities of K, P and R. METHODS: Hemidiaphragm-phrenic nerve preparations were obtained from male Sprague-Dawley rats (150-250 g). Preparations were bathed in Krebs' solution ([mM]:NaCl 118, KCl 5, CaCl2 2.5, NaHCO3 30, KH2PO4 1, MgCl2 1 and glucose 11), maintained at 32degreeC and aerated with a mixture of 95% O2 and 5% CO2. isometric forces generated in response to 0.1 Hz, and 50 Hz for 1.9 seconds with supramaximal stimulation (0.2 ms, rectangular) to the phrenic nerve, were measured using a force transducer. The effects on single twitch tension (ST) and peak tetanic tension (PTT) were calculated as % reduction. The effects on tetanic fade (TF) were calculated as % increase. K, P and R were added to the tissue bath to achieve the desired bath concentrations. EC5, EC25, EC50, EC75, and EC95 for ST, PTT and TF were calculated using a probit model. The antagonism indices of neuromuscular blockades by calcium (5 mM), 3,4-diaminopyridine (3,4-DAP) (10muM), and neostigmine (N) (250 nM) were assessed at 85+/-5% reduction (or increase). RESULTS: The potencies of ST, PTT and TF were 5.49, 5.73 and 6.30 (mM) for K, 1.90, 1.67 and 1.31 (mM) for P, and 10.81, 5.27 and 4.41 (muM) for R. The correlation between ST, PTT and TF varied for K, P and R. Neuromuscular blockades of K were reversed similarly by calcium and 3,4-DAP, and partially by N, whilst those of P were not, and ST reduction of R was reversed by 3,4-DAP (98%) and PTT and TF of R were partially reversed by 3,4-DAP and N. CONCLUSiONS: it is considered probable that K inhibits acetylcholine release and noncompetitively blocks postsynaptic sites, P postsynaptically depresses the neuromuscular transmission with acetylcholine receptor-ion channel block and that R has not only a selective presynaptic effect but also a postsynaptic block effect.

Efficacy of flouroquinolone [Pefloxacin] on mycoplasma infection in chickens

The efficacy of the newer flouroquinolone [pefloxacin] given alone or in-combination with levamisole against Mycoplasma gallisepticum [MG] in experimentally infected chickens was investigated. Two hundred one day old chicks were used. The use of levamisole as a non-specific immunostimulant with pefloxacin 10 mg/kg body weight showed good curative effect which was indicated by decrease in mortality rates, increase in the body weight gain, high feed conversion as well as low rate of re-isolated MG and low titre in HIT as compared with those received pefloxacin alone 10 mg/kg body weight or levamisole 2.5 mg/kg body weight alone

Multidrug resistant salmonella typhi in Gujranwala

One hundred and thirty strains of Salmonella typhi were isolated from the blood of patients clinically suspected to be suffering from typhoid fever for the 5-year period from Jan 1995 to Dec. 2000. The blood cultures were taken in brain heart infusion broth. The isolates were identified based on morphological, biochemical and serological characteristics. The susceptibility of the strains was tested by disc diffusion tests as per standard methods. A high level of resistance to first-line drugs was observed. 43.85 percent strains of S. typhi revealed resistance to chloramphenicol, 42.3 percent to ampicillin, 46.15 percent to co-trimoxazole, 37.69 percent to Amoxicillin, 6.15 percent to ciprofloxacin, 0.77 percent to pefloxacin, enoxacin, and ofloxacin. No resistance to ceftriaxone and cefotaxime was observed

A Case of Drug Eruption Caused by Quinolones / 대한피부과학회지

The drug eruptions caused by quinolones are rarely reported. The patient took norfloxacin for several days prior to admission and was admitted due to systemic erythematous maculopapular eruptions. For the treatment of urinary tract infection, he was treated with pefloxacin via intravenous route, and then the symptom of drug eruption became aggravated. After termination of treatment, the symptom reduced. The causative agent was identified by intradermal test and confirmed by accidental rechallenge with levofloxacin which is an another quinolone. We report herein the case of drug eruption caused by several quinolones which was confirmed by intradermal test and incidental administration of the drug.

Quinolone induced arthropathy in adolescents and adults

To ascertain the incidence, clinical pattern and outcome of arthropathy in adolescents and adults induced by different quinolones. Cases of enteric fever treated with different quinolones having no previous history of joint disorders who developed arthropathy and improved well with conservative treatment were labelled as quinolone induced arthropathy. In-patients of Rajshahi and Khulna Medical College Hospitals and private clinics of the authors. Four hundred and three cases of enteric fever were treated with various quinolones during the period January 1995 to June 1999. Main outcome measures: Clinical characteristics, incidence, relation with quinolones used and outcome of quinolone induced arthropathy. The age of the 403 [males 260 and females 143] patient ranged from 13-60 years. They were treated with either ciprofloxacin, pefloxacin or ofloxacin. Out of them 31 [7.7%] patients developed arthropathy. It was highest with pefloxacin [12.5%]. The arthropathy was maximum [11.8%] in the age group of 11-20 years. Patterns of arthropathy were oligoarthropathy [23], polyarthropathy [7] and monoarthropathy [1]. Although quinolones use is not advocated in children for development of arthropathy, it also occurs in adolescents and adults in considerable number of cases. It improves well with conservative treatment without residual effect

Intraventricular Pefloxacine Therapy for a Cerebral Ventriculitis by Enterobacter Aerogenes: Case Report

No abstract available.

Infección y neutropenia: pefloxacina versus amikacina-ceftazidima como terapia antimicrobiana empírica inicial / Infection and neutropenia, pefloxacine vs. amikacine-ceftazidime as an initial antimicrobial empirical therapy

Objetivo. Comparar la eficacia y eficiencia de la pefloxacina vs la amika-cina-ceftazidima, seguidas de vancomicina, como terapia empírica en episodios de infección y neutropenia. Material y métodos. El estudio fue prospectivo. Se incluyeron pacientes mayores de 15 años, con temperatura superior a 38ºC y concentración de neutrófilos menor de 1 x 10 a la 9/L. La asignación a la ramas de tratamiento se hizo al azar. Un grupo recibió pefloxacina, el otro amikacina y ceftazidima. En ambas rama, si la fiebre persistió por 72 h se agregó vancomicina; si la fiebre persistió por 10 días, se agregó anfotericina B. Resultados. 38 pacientes evaluables (17/21) en ambas ramas fueron comparables en patología básica, aplicación de quimioterapia, neutrófilos iniciales, neutropenia máxima, neutrófilos finales, focos infecciosos y gérmenes aislados. La curación se observó en 87 y 89 por ciento de los casos, respectivamente (p=0.52). La duración promedio de la fiebre fue de 6.1 y 5.8 días (p=0.84). Conclusión. La pefloxacina y la amikacina-ceftazidima tienen eficacia y eficiencia comparables como antibióticos iniciales en episodios de infección y neutropenia, usados como tratamiento antimicrobiana empírica

A comparative study of anxiogenic effects of fluoroquinolones in rats.

The present study compares the anxiogenic effects of three fluoroquinolones namely ciprofloxacin, ofloxacin and pefloxacin in rats using elevated plus-maze. The rats were treated with 12.5 mg/kg and 25 mg/kg of ciprofloxacin, ofloxacin or pefloxacin and then tested in elevated plus-maze half an hour later, for a period of 5 min. All the three fluoroquinolones decreased the time spent in open arm considerably. This decrease was statistically significant only with the higher doses of ciprofloxacin and ofloxacin (P < 0.05). Mean time spent in closed arm was increased by all the test drugs in both the doses. Increase was statistically significant with both the doses of ofloxacin (P < 0.05). Mean time spent in closed arm was increased by all the test drugs in both the doses. Increase was statistically significant with both the doses of ofloxacin (P < 0.05, P < 0.01 respectively) and higher doses of ciprofloxacin and pefloxacin (P < 0.01, P < 0.05 respectively). The number of entries in open arm and closed arm were decreased by both the doses of the three fluoroquinolones used in the study. The reduction in total number of arm entries by ciprofloxacin, ofloxacin and pefloxacin in both the doses was highly significant. The results suggest definite anxiogenic potential of fluoroquinolones.